Development of a protocol for genetic transformation of Malus spp
نویسندگان
چکیده
A protocol to produce transgenic shoots of Malus X domestica cv Greensleaves was optimized using two gene constructs previously used create parthenocarpic tomato, Ino-IaaM and DefH9-IaaM. The aim obtain sufficient nº for in vitro multiplication, transfer soil, grafting testing parthenocarpy the next years. We investigated effects modifications a previous published protocol: 1) co-transformation with an Agrobacterium containing “VIP” genes construct 2) different hormones or hormone combinations. More shoot regeneration obtained combination three (BA:NAA:TDZ) during co-cultivation instead IBA no performed VIP gene. For DefH9-IaaM transgene, 21.04% achieved this treatment 8.95% “IBA treatment” 4.42% treatment. occurred only Experiments confirmed results shown transgene. regenerated were multiplied selective media kanamycin roots obtained.
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ژورنال
عنوان ژورنال: Caryologia
سال: 2021
ISSN: ['0008-7114', '2165-5391']
DOI: https://doi.org/10.36253/caryologia-1248